SubtiBank SubtiBank
yjbO [2018-02-13T15:32:04.000Z]
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yjbO [2018-02-13T15:32:04.000Z]

similar to pseudouridylate synthase
locus
BSU11620
pI
6.55
mw
31.32 kDa
protein length
283 aa Sequence Blast
gene length
849 bp Sequence Blast
function
RNA modification
product
putative pseudouridylate synthase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.3|rRNA modification and maturation/ based on similarity]

    • Gene

    Coordinates
    1,238,523 → 1,239,374

    The protein

    Catalyzed reaction/ biological activity

  • RNA + uridine --> RNA pseudouridine (according to Swiss-Prot)

    • Protein family

  • pseudouridine synthase rluA family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|064AE8CE6D52E35582F90654AA9548963573C05C|YhcT], [protein|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|YlyB]

    • Structure

  • [PDB|1V9F] (RluD from E. coli, 40% identical, aa 64 - 279) [pubmed|15078091]

    • Expression and Regulation

    Operons

    genes
    [gene|E5A57660B69F9E9BC52FD9C8A5E3C8786CE96728|yjbL]-[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]-[gene|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF]-[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]
    description
    [Pubmed|18067544]

    regulation

  • expressed during exponential growth
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    Biological materials

    Mutant

  • MGNA-B161 (yjbO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1160 NBRP B. subtilis, Japan]
  • BKE11620 (Δ[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG, downstream forward: _UP4_GAGAATCATTGATTCTCTCC
  • BKK11620 (Δ[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG, downstream forward: _UP4_GAGAATCATTGATTCTCTCC

    • References

  • 18067544,15078091